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AnaSpec 520 mmp fret substrate
520 Mmp Fret Substrate, supplied by AnaSpec, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/520+mmp+fret+substrate/520+mmp+fret+substrate+xiv/pm40624143-163-6-9
Average 90 stars, based on 1 article reviews
520 mmp fret substrate - by Bioz Stars, 2026-10
90/100 stars

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Incubation:

Article Title: Matrix metalloproteinase-2 as a novel regulator of glucose utilization by adipocytes.
Article Snippet: In situ zymography was performed on fixed five-micrometer gonadal WAT cross-sections hydrated with HistoClear Clearing Agent (MilliporeSigma), 100% ethanol, 90% ethanol, 70% ethanol, 20% ethanol, and then water32. .. Sections were then incubated with 520 MMP FRET Substrate (Anaspec, #AS-60568-01) in reaction buffer (50 mM Tris-HCl pH 7.5, 10 mM CaCL2, 150 mM NaCl, 5 μM ZnCl2) at 37 °C in dark overnight. .. The slides were mounted with ProLong Gold Antifade Mountant with DAPI (Invitrogen, #P36931) before image acquisition on a EVOS M5000 (ThermoFisher).



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